Cuid 2: Airíonna Frith-athlastacha Agus Frithocsaídeacha de Mhíle Muiléad (Eleusine Coracana (L.) Gaertn.) Cineálacha Saothraithe i Srí Lanca

Le haghaidh tuilleadh eolais. teagmháiltina.xiang@wecistanche.com

Athlasadhfreagra imdhíonachta ár gcorp chun ionfhabhtú a chomhrac agus fíochán damáiste a dheisiú; is próiseas féin-leighis agus cneasaithe é a spreagann galar áirithe inár gcorp.

Tá go leor galair an chorp daonna bainteach le athlasadh. Mar shampla, léirigh staidéir gurb é bunchúis galar croí athlasadh artaireach, mar shampla osteoarthritis éagsúla, athlasadh gínéiceolaíoch, gastroenteritis, heipitíteas ainsealach, cholecystitis, nephritis, niúmóine, agus fiú riníteas. , keratitis, otitis media ... fiú ailse, is féidir a rá go bhfuil beagnach gach galar ainsealach a bhaineann le athlasadh.

Nuair a ionsaíonn baictéir dhochracha nó víreas sinn, tosaíonn córas bailíochtaithe ár gcorp isteach agus troideann sé ar ais; nó má sprainimid ár rúitín, tosaíonn ár gcóras athlastach isteach chun an fíochán damáiste a dheisiú. Gan an córas athlastach, thiocfadh linn bás a fháil go tapa.

Staidéir turgnamhacha Tá sé léirithe go echinacea-saibhirCistanche deserticolatá éifeacht feabhsaithe maith ag sliocht ar lucha colitis. Tá éifeacht chosanta ag na comhdhúile fenethylgly atá scoite ó Cistanche ar chealla athlastacha de bharr aonocsaíde carbóin. Tá éifeacht coisctheach maith ag glucosides iomlána Cistanche ar an secretion aonocsaíde carbóin i gcealla athlastacha, agus léiríonn na torthaí go bhfuil éifeacht frith-athlastach aige. Thairis sin, tá Cistanche ina tonic maith ó am ársa, agus níl aon taifead ar thocsaineacht, rud a chruthaíonn a sábháilteacht go hiomlán.

3. Torthaí agus Plé

3.1.Airíonna Frithocsaídeacha de Chineálacha Muiléad Méar

3.1.1.Ábhar Iomlán Feanólach agus Iomlán Flavonoid.Cuirtear TPC agus TFC de shleachta eatánólacha agus meatánólacha de chineálacha muiléad méar Ravi, Rawana, agus Oshadha i láthair i dTábla 1. Bhí difríochtaí suntasacha ann (P<0.05)among the="" varieties="" and="" between="" the="" extracts.="" methanolic="" extracts="" had="" significantly=""><0.05)tpcs and="" tfcs="" compared="" to="" ethanolic="" extracts="" in="" all="" finger="" millet="" varieties.="" among="" the="" three="" varieties,="" oshadha="" had="" a="" significantly="" high=""><0.05)tpc, and="" ravi="" had="" a="" significantly=""><0.05 )tfc.="" there="" were="" no="" significant="" differences(p≥0.05)between="" the="" tfcs="" of="" rawana="" and="">

diúltach suntasach (P<0.05) correlations="" were="" observed="" between="" tpcs="" and="" ics.="" values="" of="" dpph="" (r="-0.602)" and="" abts="" cation(r="-0.836)radical" scavenging="" activities="" of="" all="" extracts.in="" addition,a="" significant="" positive=""><0.05) correlation="" was="" observed="" between="" tpcs="" and="" orac="" values(r="0.787)" of="" the="" extracts.="" these="" correlations="" indicated="" the="" involvement="" of="" finger="" millet="" phenols="" in="" radical="" scavenging="" activities.="" tpcs="" of="" the="" extracts="" positively="" correlated="" with="" frap="" values(r="0.876,"><0.05)and fic="" activities(r="0.473,"><0.05)of the="" extracts="" indicating="" the="" ability="" of="" finger="" millet="" phenols="" to="" reduce="" ferric="" ions="" and="" chelate="" ferrous="" ions.="" there="" were="" significant=""><0.05)correlations between="" tfcs="" and="" ic5="" values="" of="" dpph(r="-0.802)and" abts="" cation(r="-0.471)radical" scavenging="" activities="" of="" the="" extracts.="" besides,="" tfcs="" positively="" correlated="" with="" fic="" activities(r="0.689,"><0.05)of the="" extracts,="" suggesting="" the="" involvement="" of="" finger="" millet="">flavonoids i radacacha scavenging agus iain fheiriúla chelating.

Thug Abeysekera et al tuairisc ar TPCanna de ghránaigh choitianta [30]. Bhí sleachta eatánólacha sa raon ó 59.78 go 113.56mg GAE in aghaidh 100g plúr ar bhonn meáchain thirim. B'ionann TPCanna sleachta meatánóil eorna, arbhar, cruithneachta agus coirce 93.61,75.10,71.22, agus 17.24mg CAE in aghaidh 100g plúr, faoi seach, ar bhonn meáchain thirim. B'ionann TPCanna sleachta eatánólach eorna, arbhar, cruithneachta agus coirce 68.69,52.10, 78.05, agus 15.72mg CAE in aghaidh an 100g plúr, faoi seach, ar bhonn meáchain thirim. Mar sin, i gcomparáid leis na torthaí thuasluaite, tá ábhar feanólach ard ag cineálacha muiléad méar Ravi, Rawana, agus Oshadha ná gránaigh a úsáidtear go coitianta mar rís, cruithneacht, arbhar, eorna agus coirce. Is eol go bhfuil comhdhúile feanólacha láidir frith-ocsaídeoirí, agus dá bhrí sin, is féidir aon fhíochán planda ina bhfuil na comhdhúile seo a úsáid mar fhrithocsaídeoirí nádúrtha éifeachtacha [2]. Mar thoradh air sin, léirigh an t-ábhar ard feanólach cumas frithocsaídeach na gcineálacha Ravi, Rawana agus Oshadha.

3.1.2.DPPH agus ABTS Gníomhaíochtaí Snámha Radacacha. Difríochtaí suntasacha (P<0.05) were="" observed="" in="" both="" dpph="" and="" abts="" cation="" radical="" scavenging="" activities="" of="" ravi,="" rawana,="" and="" oshadha="" varieties="" and="" between="" the="" extracts="" (table="" 2).="" methanolic="" extracts="" of="" the="" three="" varieties="" showed="" significantly=""><0.05)dpph and="" abts="" cation="" radical="" scavenging="" activities="" when="" compared="" to="" ethanolic="" extracts.="" ravi="" and="" oshadha="" varieties="" showed="" significantly="" high=""><0.05)dpph and="" abts="" cation="" radical="" scavenging="" activities="" when="" compared="" to="" rawana.="" all="" extracts="" showed="" dose-dependent="" dpph="" and="" abts="" cation="" radical="" scavenging="" activities.="" in="" dpph="" radical="" scavenging="" assay,="" the="" methanolic="" extract="" of="" oshadha="" had="" the="" lowest="" ics="" value="" indicating="" the="" highest="" activity,="" and="" in="" abts="" cation="" radical="" scavenging="" assay,="" the="" methanolic="" extract="" of="" ravi="" had="" the="" lowest="" ics="" value="" indicating="" the="" highest="">

Deimhneach suntasach (P<0.05)correlations were="" observed="" between="" ic="" values="" of="" dpph="" and="" abts="" cation="" radical="" scavenging="" activities="" of="" the="" extracts(r="0.750)indicating" that="" the="" finger="" millet="" extracts="" had="" comparable="" activities="" in="" the="" two="" assays.="" the="" compounds="" present="" in="" finger="" millet="" extracts="" that="" can="" scavenge="" dpph="" radicals="" may="" be="" capable="" of="" scavenging="" abts="" cation="" radicals="" as="" well.="" almeida="" et="" al.[31]="" have="" observed="" a="" similar="" correlation="" when="" studying="" the="" antioxidant="" activities="" of="" brazilian="" fruit="" extracts.="" suriano="" et="" al.="" [32]="" also="" have="" reported="" a="" similar="" correlation="" in="" barley="" varieties,="" and="" they="" have="" attributed="" it="" to="" the="" same="" reaction="" mechanism="" on="" which="" both="" assays="" rely.="" a="" good="" antioxidant="" should="" be="" capable="" of="" quenching="" free="" radicals="" [33,34].="" therefore,="" the="" ability="" to="" scavenge="" dpph="" and="" abts="" cation="" radicals="" indicated="" the="" antioxidant="" potential="" of="" ravi,="" rawana,="" and="" oshadha="" finger="" millet="">

3.1.3.Cumas ionsúcháin radacach ocsaigine. Bhí difríochtaí suntasacha ann (P<0.05) in="" scavenging="" oxygen="" radicals="" among="" the="" ravi,="" rawana,="" and="" oshadha="" varieties(table="" 3), and="" the="" orac="" value="" of="" oshadha="" was="" significantly="" higher=""><0.05)than that="" of="" the="" ravi="" and="" rawana="" varieties.="" in="" raw-ana,="" the="" ethanolic="" extract="" showed="" a="" significantly="" high=""><0.05) orac="" value="" when="" compared="" to="" the="" methanolic="" extract.="" there="" were="" no="" significant="" differences="" (p≥0.05)between="" the="" orac="" values="" of="" ethanolic="" and="" methanolic="" extracts="" in="" the="" ravi="" and="" oshadha="">

Tuairiscíodh luachanna ORAC ar ghránaigh choiteanna i staidéir roimhe seo [30]. Bhí luachanna ORAC ar eastóscáin meatánóil de chineálacha ríse bán agus dearg sa raon ó 150.61 go 439.64mg coibhéis Trolox in aghaidh 100g plúr ar bhonn meáchain thirim, agus bhí luachanna ORAC d’eastóscáin eatánóil sa raon ó 108.32 go 272.08mg coibhéis Trolox in aghaidh an. 100g de phlúr ar bhonn meáchain thirim. Ba iad luachanna ORAC na n-eastóscáin meatánóil eorna, cruithneachta, arbhar, agus coirce ná 357.51, 322.00,249.91, agus coibhéisí 50.71mg Trolox in aghaidh an 100g plúir, faoi seach, ar bhonn meáchain thirim. B'ionann luach na n-eastóscán eatánólach d'eorna, cruithneacht, arbhar agus coirce agus 244.33,263.90,84.04, agus coibhéisí 73.20mg Trolox in aghaidh an 100g plúir, faoi seach, ar bhonn meáchain thirim. Mar sin, i gcomparáid leis na torthaí thuasluaite, tá cumas níos airde ag cineálacha muiléad méar Ravi, Raw-ana, agus Oshadha fréamhacha sárocsaile a scamhadh ná na gránaigh eile a chaitear go coitianta mar rís, cruithneacht, agus arbhar, eorna agus coirce. Ós rud é go bhfuil cumas sliocht chun fréamhacha fiseolaíocha cosúil le fréamhacha perocsaile a scaveneáil ina tháscaire tábhachtach dá chumas frithocsaídeach [33,34], léirigh na torthaí seo cumas frithocsaídeach na gcineálacha Ravi, Rawana, agus Oshadha.

3.1.4.Gníomhaíocht Chealaithe ian Neamhfheiriúil. Bhí difríochtaí suntasacha ann (P<0.05) in="" fic="" activities="" of="" ravi,="" rawana,="" and="" oshadha="" varieties="" and="" between="" the="" extracts="" (table="" 3).="" methanolic="" extracts="" showed="" significantly="" high=""><0.05)activities when="" compared="" to="" ethanolic="" extracts="" of="" all="" finger="" millet="" varieties.="" therefore,="" methanol="" extraction="" may="" be="" more="" efficient="" compared="" to="" ethanol="" extraction="" when="" extracting="" com-pounds,="" with="" ficability,="" from="" finger="" millet="" varieties.="" ferrous="" ions="" can="" initiate="" and="" spread="" many="" radical="" generating="" reactions,="" and="" consequently,="" chelating="" ferrous="" ions="" are="" important="" to="" prevent="" redox-active="" metal="" catalysis-associated="" ros="" generating="" reactions[35].="" therefore,="" the="" ability="" to="" chelate="" ferrous="" ions="" reflected="" the="" antioxidant="" potential="" of="" the="" ravi,="" rawana,="" and="" oshadha="">

3.1.5.Cumhacht Frithocsaídeach Laghdaithe Ferric. Bhí difríochtaí suntasacha ann (P<0.05) in="" frap="" values="" of="" ravi,="" rawana,="" and="" oshadha="" varieties="" and="" between="" the="" extracts(table="" 3).="" methanolic="" extracts="" showed="" significantly="" high=""><0.05)frap values="" when="" compared="" to="" ethanolic="" extracts.="" among="" the="" three="" varieties,="" oshadha="" showed="" a="" significantly="" high=""><0.05)frap value="" when="" compared="" to="" ravi="" and="" rawana.="" since="" the="" reducing="" capacity="" of="" an="" extract="" is="" an="" important="" indicator="" of="" its="" antioxidant="" capacity="" [2],="" these="" results="" provided="" evidence="" for="" the="" antioxidant="" potential="" of="" ravi,="" raw-ana,="" and="" oshadha="">

Bhí luachanna FRAP ar eastóscáin meatánóil de chineálacha ríse bán agus dearg sa raon ó 61.79 go 743.18mg coibhéisí Trolox in aghaidh 100g plúir ar bhonn meáchain thirim, agus bhí luachanna FRAP d’eastóscáin eatánólacha sa raon ó 139.36 go 255.34mg coibhéisí Trolox in aghaidh an 100g plúir thirim bonn meáchain. B'ionann luachanna FRAP d'eastóscáin meatánóil eorna, cruithneachta, arbhar, agus coirce agus 199.45,177.95,81.06, agus 61.74mg coibhéisí Trolox in aghaidh 100g plúir, faoi seach, ar bhonn meáchain thirim. Ba iad luachanna FRAP na n-eastóscanna eatánólach eorna, cruithneachta, arbhar, agus coirce ná 107.21,84.27,49.05, agus coibhéisí 26.60mg Trolox in aghaidh 100g plúr, faoi seach, ar bhonn meáchain thirim [30]. Mar sin, nuair a chuirtear i gcomparáid leis na torthaí thuasluaite, tá cumas níos airde ag cineálacha muiléad méar Ravi, Rawana agus Oshadha chun hiain ferric a laghdú i gcomparáid le rís, cruithneacht, arbhar, eorna agus coirce. Ós rud é go bhfuil laghdú ar chumas sliocht ina tháscaire tábhachtach ar a chumas frithocsaídeach [2], léirigh an cumas chun hiain ferric a laghdú acmhainneacht frithocsaídeach na gcineálacha Ravi, Rawana, agus Oshadha.

3.2.Airíonna Frith-athlastacha de Chineálacha Muiléad Finger

3.2.1.A5-Gníomhaíocht Chosc ar Einsím LOX.A5-Tugtar gníomhaíochtaí coisctheacha einsímeacha LOX de na heastóscáin eatánólacha agus meatánólacha de chineálacha Ravi, Rawana, agus Oshadha i dTábla 4. Tá an dá eastóscadh de na cineálacha muiléad trí mhéar in ann gníomh catalaíoch an A a chosc. 5-Einsím LOX ar bhealach dáileog-spleách. Léirigh sleachta meatánóil de na trí chineál go suntasach (P<0.05) high="" a5-lox="" enzyme="" inhibitory="" activities="" when="" compared="" to="" ethanolic="" extracts.="" among="" the="" six="" finger="" millet="" extracts,="" the="" methanolic="" extract="" of="" oshadha="" had="" the="" lowest="" icso="" value="" indicating="" the="" highest="" a5-lox="" enzyme="" inhibitory="">

Comhghaolaíodh luachanna ICC de ghníomhaíochtaí coisctheacha einsíme A{{0}LOX de na heastóscáin agus TPCanna (r=-0.788, P<0.05)and tfcs(r="-0.399,"><0.05)of the="" extracts,="" indicating="" the="" involvement="" of="" finger="" millet="" phenols,="" including="" flavonoids,="" in="" inhibiting="" the="" catalytic="" action="" of="" a5-lox="" enzyme.="" perera="" et="" al.="" [2]="" have="" observed="" a="" similar="" correlation="" in="" sri="" lankan="" medicinal="" plants,="" and="" they="" have="" reported="" that="" anti-a5-lox="" activity="" is="" proportional="" to="" the="" polyphenol="" and="" flavonoid="" contents.="" this="" is="" supported="" by="" the="" fact="" that="" most="" of="" the="" a5-lox="" enzyme="" inhibitors="" are="" phenols,="" and="" furthermore,="" the="" most="" potent="" a5-lox="" enzyme="" inhibitors,="" including="" baicalein,="" are="">

Bhí comhghaolta dearfacha láidre idir luachanna IC de ghníomhaíochtaí coisctheacha einsíme A{{{{{0}} agus luachanna ICs0 DPPH(r=0.840, P<0.05)and abts="" cation="" (r="0.660,"><0.05)radical scavenging="" activities="" of="" all="" extracts.="" these="" correlations="" indicated="" the="" involvement="" of="">frithocsaídeoirí, atá i láthair i sleachta muiléad méar, chun an einsím A5-LOX a chosc trí na hidirmheánacha radacacha a ghintear le linn ghníomhaíocht chatalaíoch an A5-LOXenzyme a scavening. Tá adamh iarainn neamhheme i láthair i suíomh gníomhach lipoxygenases. Is éard atá i gceist le imoibriú catalaíoch lipoxygenases ná ocsaídiú aon-leictreon ag adamh iarainn an tsuímh ghníomhach a aistríonn idir stáit Fe2 móide agus Fe3 móide redox. Mar sin, is féidir le móilíní le feidhmiúlachtaí laghdaithe ian iarainn-chelating agus ferric feidhmiú mar choscóirí láidre A{5-LOX[2,37]. Sa staidéar seo, tugadh faoi deara freisin comhghaolta diúltacha láidre idir na luachanna IC de A{{9} }Gníomhaíochtaí coisctheacha einsím LOX agus luachanna FRAP (r=-0.873, P<0.05)as well="" as="" fic="" activities(r="-0.752,"><0.05)of all="" extracts="" suggesting="" that="" reducing="" the="" ferric="" ions="" and="" chelating="" the="" ferrous="" ions="" as="" potential="" mechanisms="" of="" finger="" millet="" extracts="" in="" inhibiting="" the="" catalytic="" action="" of="" the="" a5-lox="">

3.2.2. Gníomhaíocht Chosc ar Einsím XO.Bhí eastóscáin eatánólacha agus meatánólacha de chineálacha muiléad méar Ravi, Rawana, agus Oshadha in ann cosc ​​a chur ar ghníomh catalaíoch na heinsíme xanthine oxidase ar bhealach dáileog-spleách a léirigh anfrith-athlastachpoitéinseal na dtrí chineál (Tábla 4). Léirigh sleachta meatánóil go suntasach (P<0.05)high xo="" enzyme="" inhibitory="" activities="" when="" compared="" to="" ethanolic="" extracts.="" among="" the="" six="" finger="" millet="" extracts,="" the="" methanolic="" extract="" of="" oshadha="" had="" the="" lowest="" icso="" value="" indicating="" the="" highest="" xo="" enzyme="" inhibitory="">

Léirigh luachanna ICC de ghníomhaíochtaí coisctheacha einsím XO de gach sleachta go suntasach (P<0.05) negative="" correlations="" with="" tpcs(r="-0.744)," tfcs(r="-0.489)," frap="" values="" (r="-0.793)," and="" fic="" activities(r="-0.906)indicating" the="" involvement="" of="" antioxidants,="" which="" are="" present="" in="" finger="" mil-let="" extracts,="" in="" inhibiting="" the="" catalytic="" action="" of="" the="" enzyme.="" during="" the="" catalytic="" action="" of="" xo,="" superoxide="" anions="" and="" hydrogen="" peroxide="" are="" formed="" [38].="" the="" significant=""><0.05)positive correlations="" between="" ic="" values="" of="" xo="" enzyme="" inhibitory="" activities="" and="" ics.values="" of="" dpph="" (r="0.826)" and="" abts="" cation="" (r="0.790)radical" scavenging="" activities="" of="" all="" extracts="" suggested,="" scavenging="" radical="" intermediates="" including="" ros,="" which="" are="" generated="" during="" the="" catalytic="" action="" of="" the="" enzyme,="" as="" a="" potential="" mechanism="" of="" xo="" enzyme="">

3.2.3.Gníomhaíocht Chosc ar Einsím Hyaluronidase. Bhí sleachta eatánólacha agus meatánólacha de chineálacha muiléad méar Ravi, Rawana, agus Oshadha in ann cosc ​​a chur ar ghníomh catalaíoch na heinsíme hyaluronidase (Fíor 1). I measc na dtrí chineál, léirigh Oshadha an ghníomhaíocht coisctheach einsím hyaluronidase is airde cé nach raibh sé suntasach go staitistiúil (P Níos mó ná nó cothrom le 0.05) i gcomparáid leis an dá chineál eile. Ní raibh aon difríochtaí suntasacha (P Níos mó ná nó cothrom le 0.05) idir ghníomhaíochtaí coisctheacha einsím hyaluronidase de na sé sleachta, agus bhí siad suntasach (P<0.05)lower than="" the="" hyaluronidase="" enzyme="" inhibitory="" activity="" of="" the="" reference="" standard,="" tannic="" acid="" which="" showed="" 99.16%="" inhibition="" at="" 0.5mg/ml="" concentration.="" girish="" et="" al.="" [7]="" and="" sahasrabudhe="" and="" deodhar="" [27]="" have="" reported="" that="" polyphenols="" are="" good="" hyaluronidase="" inhibitors.="" furthermore,="" the="" reference="" standard="" used="" in="" this="" assay,="" tannic="" acid,="" is="" also="" a="" type="" of="" polyphenol="" [27].="" in="" the="" present="" study="" also,="" hyaluronidase="" enzyme="" inhibitory="" activities="" of="" the="" extracts="" showed="" a="" positive="" correlation="" with="" tpcs(r="0.387)," confirming="" the="" involvement="" of="" finger="" millet="" phenols="" in="" inhibiting="" the="" catalytic="" action="" of="" the="" hyaluronidase="">

3.2.4.Gníomhaíocht Chosc ar Phléascadh Ocsaídeach. Rinneadh measúnú ar chumas na n-sleachta eatánólacha agus meatánólacha de chineálacha muiléad méar Ravi, Rawana, agus Oshadha chun an pléasctha ocsaídiúcháin zymosan-spreagtha san fhuil iomlán daonna a chosc, agus léirigh na torthaí go bhfuil an dá sliocht de na cineálacha muiléad trí mhéar in ann. cosc a chur ar phléasc ocsaídiúcháin san fhuil dhaonna ar fad ar bhealach a bhraitheann ar dháileog (Tábla 4). Léirigh sleachta meatánóil go suntasach (P<0.05) high="" inhibition="" when="" compared="" to="" ethanolic="" extracts.="" when="" compared="" with="" ibuprofen,="" methanolic="" extracts="" had="" a="" moderate="" oxidative="" burst="" inhibitory="" potential="" in="" whole="" human="" blood.="" all="" extracts="" were="" further="" evaluated="" for="" their="" oxidative="" burst="" inhibitory="" activity="" in="" isolated="" polymorphonuclear="" neutrophils(table="" 4).="" similar="" to="" the="" results="" of="" the="" study="" with="" whole="" human="" blood,="" all="" extracts="" showed="" dose-dependent="" oxidative="" burst="" inhibitory="" activities="" in="" poly-morphonuclear="" neutrophils,="" and="" methanolic="" extracts="" exhibited="" significantly=""><0.05)high inhibition="" when="" compared="" to="" ethanolic="" extracts.="" according="" to="" the="" results="" of="" the="" present="" study,="" both="" ethanolic="" and="" methanolic="" extracts="" of="" finger="" millet="" varieties="" exhibited="" a="" high="" oxidative="" burst="" inhibition="" in="" isolated="" polymorphonuclear="" neutrophils="" when="" compared="" with="" ibuprofen.="" methanolic="" extracts="" of="" ravi,="" rawana,="" and="" oshadha="" exhibited="" 8.5,5.2,="" and="" 5.6="" times="" higher="" activities,="" respectively,="" for="" oxidative="" burst="" inhibition="" in="" human polymorphonuclear="" neutrophils="" when="" compared="" to="" ibuprofen.="" this="" may="" be="" attributed="" to="" the="" significantly="" high=""><0.05) tpcs="" and="" tfcs(table="" 1)="" of="" methanolic="" extracts="" of="" the="" three-finger="" millet="">

Fuarthas amach go raibh TPCanna na n-sleachta comhghaolaithe go diúltach le luachanna IC5 de chosc pléasctha ocsaídiúcháin san fhuil iomlán dhaonna (r=-0.431) agus neutrophils polamorphonuclear daonna(r=-0.528, P<0.05). similarly,="" tfcs="" of="" the="" extracts="" were="" found="" to="" be="" negatively="" correlated="" with="" ic5so="" values="" of="" oxidative="" burst="" inhibition="" in="" whole="" human="" blood="" (r="-0.715,"><0.05)and human="" polymorphonuclear="" neutrophils(r="-0.619,"><0.05). these="" correlations="" indicated="" the="" involvement="" of="" finger="" millet="" phenols,="" including="" flavonoids,="" in="" oxidative="" burst="" inhibition.="" the="" significant=""><0.05)negative correlations="" between="" frap="" values="" of="" the="" extracts="" and="" oxidative="" burst="" inhibitory="" activity="" in="" whole="" human="" blood="" (r="-0.541)" and="" human="" polymorphonuclear="" neutrophils="" (r="-0.602)as" well="" as="" fic="" activities="" of="" the="" extracts="" and="" ics.values="" of="" oxidative="" burst="" inhibition="" in="" whole="" human="" blood(r="-0.985)and" human="" polymorphonuclear="" neutrophils(r="-0.959)suggested" that="" antioxidant="" compounds="" which="" are="" present="" in="" the="" finger="" millet="" extracts="" are="" responsible="" for="" inhibiting="" the="" oxidative="" burst.="" there="" were=""><0.05) positive="" correlations="" between="" ics="" values="" of="" abts="" cation="" radical="" scavenging="" activities="" and="" icsovalues="" of="" oxidative="" burst="" inhibitory="" activities="" in="" whole="" human="" blood="" (r="0.556)" and="" human="" polymorphonuclear="" neutrophils(r="0.652).In" addition,="" there="" were="" strong="" positive="" correlations="" between="" ic="" values="" of="" dpph="" radical="" scavenging="" activities="" and="" icsvalues="" of="" oxidative="" burst="" inhibitory="" activities="" in="" whole="" human="" blood(r="0.889,"><0.05)and human="" polymorphonuclear="" neutrophils(r="0.815,"><0.05 ).="" these="" correlations="" indicated="" scavenging="" radicals="" as="" the="" prominent="" mechanism="" in="" oxidative="" burst="" inhibition.="" antioxidants,="" which="" have="" the="" potential="" to="" inhibit="" ros-induced="" oxidative="" burst,="" can="" serve="" as="" effective="" anti-inflammatory="" agents="" [1,="" 8].="" therefore,="" these="" results="" provided="" evidence="" for="" the="" anti-inflammatory="" potential="" of="" ravi,="" rawana,="" and="" oshadha="" varieties.="" oxidative="" burst="" inhibitory="" activities="" provided="" evidence="" for="" the="" anti-inflammatory="" potential="" of="" ravi="" rawana,="" and="" oshadha="" varieties="" while="" indicating="" the="" potential="" application="" of="" methanolic="" and="" ethanolic="" extracts="" of="" ravi,="" rawana,="" and="" oshadha="" finger="" millet="" varieties="" in="" the="" prevention="" and="" management="" of="" ros-induced="" inflammatory="" conditions="" as="" natural="" sources="" of="" anti-inflammatory="" drug="">

4. Conclúidí

Is é seo an chéad staidéar a thaispeánann airíonna frith-athlastacha in vitro aon sliocht d’aon chineál muiléad méar a shaothraítear i Srí Lanca ag baint úsáide as A5-LOX, XO, hyaluronidase, agus measúnachtaí coisctheacha pléasctha ocsaídiúcháin.A5-LOX. agus léirigh gníomhaíochtaí coisctheacha einsím xanthine oxidase de chineálacha muiléad méar Ravi, Rawana, agus Oshadha an cur i bhfeidhm féideartha chun neamhoird athlastacha éagsúla a chosc in éineacht le gníomhartha catalaíocha einsímí A{5-LOX agus XO. Léirigh gníomhaíochtaí coisctheacha pléasctha ocsaídiúcháin geallta san fhuil dhaonna iomlán agus neutrophils polymorphonuclear scoite go bhféadfadh eastóscáin eatánólacha agus meatánóil de chineálacha muiléad méar Ravi, Rawana, agus Oshadha a bheith ina bhfoinsí saibhir de chomhdhúile bithghníomhacha coisctheacha pléasctha ocsaídiúcháin agus dá bhrí sin léirigh siad cur i bhfeidhm féideartha Ravi, Rawana. , agus cineálacha muiléad méar Oshadha maidir le coinníollacha athlastacha de bharr ROS a chosc agus a bhainistiú. Mar gheall ar thorthaí airíonna frith-athlastacha, d’fhéadfaí cineálacha muiléad méar Ravi, Rawana agus Oshadha a úsáid go héifeachtach mar fhoinsí nádúrtha iarrthóirí drugaí frith-athlastacha, ag comhlíonadh an éilimh ar ghníomhairí frith-athlastacha núíosacha a dhíorthaítear ó fhoinsí nádúrtha. Déanfar tuilleadh staidéir cille-bhunaithe chun na torthaí seo a fhíorú.

Nocht an staidéar seo freisin airíonna frithocsaídeacha cineálacha muiléad méar Ravi, Rawana agus Oshadha. De réir na dtorthaí, tá méideanna suntasacha ard de chomhdhúile feanólacha lena n-áirítear flavonoids i gcineálacha muiléad méar Ravi, Rawana agus Oshadha. Tá an cumas ag gach ceann de na trí chineál muiléad méar scavensefréamhacha saor in aiscelena n-áirítear DPPH, ABTS cation, agus fréamhacha ocsaigine, chun iain miotail agus iain miotail chelate a laghdú a chuireann deireadh le frithghníomhartha giniúna radacacha. Léirigh na torthaí seo go bhféadfadh cineálacha muiléad méar Ravi, Raw-ana, agus Oshadha a bheith ina bhfoinsí maithe frithocsaídeoirí. Dá bhrí sin, d’fhéadfadh ról tábhachtach a bheith ag tomhaltas cineálacha muiléad méar Ravi, Rawana, agus Oshadha ar bhonn rialta i gcosc agus i mbainistíocht chothaithe na.strus ocsaídiúcháin- galair ghaolmhara. Nuair a chuirtear i gcomparáid leis na hairíonna frithocsaídeacha a thuairiscítear de ghránaigh a itear go coitianta, lena n-áirítear rís, cruithneacht, arbhar, eorna agus coirce, tá airíonna frithocsaídeacha arda ag cineálacha muiléad méir Ravi, Rawana agus Oshadha. Feidhmíonn na torthaí seo mar threoir úsáideach maidir le bianna a roghnú lena gcaitheamh go laethúil agus cuidíonn siad le cinnteoireacht a dhéanamh do thionscal an bhia chun leas a bhaint as plúr muiléad méar mar mhalairt nó mar fhorlíonadh ar phlúr gránach eile.

Le chéile, leathnaigh torthaí an staidéir seo an t-eolas atá ann faoi láthair ar airíonna bithghníomhacha na gcineálacha muiléad méar a shaothraítear agus a chaitear faoi láthair i Srí Lanca, agus leag siad béim ar a n-airíonna teiripeacha. Déanfar staidéir bhreise chun na comhdhúile feanólacha sonracha atá freagrach as gach gníomhaíocht a shainaithint.